Reporter

Part:BBa_K1114702:Design

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-17)
Revision as of 23:22, 17 September 2013 by Registry (Talk | contribs)

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BioBrick adaptation of MoClo Level 1 Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 694
    Illegal AgeI site found at 806
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a BioBrick adaptation of a Level 1 MoClo device that constitutively expresses RFP. It contains the Level 0 parts J23104_AB, BCD1_BC, E0040m_CD, and B0015_DE within the DVL1_AE backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.



Source

Level 0 MoClo parts derived from iGEM Distribution Kit

References