Reporter

Part:BBa_K1073024:Experience

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 20:20, 24 September 2013 by Kerstin K (Talk | contribs) (→‎User Reviews)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1073024

When amilGFP is expressed, the colonies on plates as well as the liquid culture become yellow.

User Reviews

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BBa_K1073024 iGEM Team Braunschweig 2013

The chromoprotein amilGFP has a yellow color. In order to avoid absoption of the chomoproteins during measurement with the photometer, the absorption and emission spectra were measured.

BSAbsorption-amilGFP.jpg

Braunschweig 2013: Absorptionspectrum of chromoprotein aeBlue in the wavelength range 400-800 nm.


absorption maximum for amilGFP: 504 nm

emission maximum for amilGFP: 515,8 nm

Although this device is working the iGEM Team Braunschweig 2013 did not use it because the yellow color is not easiely distinguishable by naked eye from the color of the media.


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