Composite

Part:BBa_K1073032

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)
Revision as of 13:20, 17 September 2013 by Registry (Talk | contribs)

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Autoinducer synthase LasI + LVA with combined eforRed chromoprotein expression cassette

This part is the basis for the expression of the Las auto inducer synthetase, which synthesizes LasI [N-(3-oxododecanoyl)-homoserine lactone]. LasI binds to the activator LasR. Together they activate the Las promoter. A constitutive expression of the chromoprotein eforRed is added to the construct in order to varify the expression of LasI synthetase. The construct has no promoter in front of the LasI synthetase gene. Therefore the user can choose the promoter himself (e.g. constitutive, induced, repressed, etc.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 838
    Illegal NheI site found at 861
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 680
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 242
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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