Composite
Part:BBa_K1073029
Designed by: iGEM Team Braunschweig 2013 Group: iGEM13_Braunschweig (2013-09-17)
Inducible ampicillin resistance cassette + LuxR expression cassette
In complex with the inducer 3-oxo-hexanoyl-HSL, the activator LuxR binds to the Lux promoter and enables expression. This construct provides all the parts required to express the gene behind the Lux promoter. Only the inducer 3-oxo-hexanoyl-HSL is missing, which usually is taken up from the media. By the regulation of the inducer's concentration in the media, the expression rate of the gene of interest (in this case ampicillin beta-lactamase) can be controlled.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1098
Illegal NheI site found at 1121 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |