Part:BBa_K1062001

Guide RNA (gRNA) target for GFP

This part is a guide RNA (gRNA) that targets GFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

In both of our projects, we used gRNAs and dCas9 as:

1) To specifically target and repress the "virulence gene" (we used flourescent proteins as a proof of concept) in our conjugation project. However, the gRNA for GFP is not used in the conjugation project since the green fluorescent protein is to represent the donor strain of the project

2) Improve past circuits with better decision making by using dCas9 instead of traditional repressors.

Conjugation Project

The GFP gRNA was not used in the Conjugation Project

Synthetic Circuit