Reporter

Part:BBa_K1067001

Designed by: Kristian Davidsen   Group: iGEM13_DTU-Denmark   (2013-09-19)
Revision as of 19:22, 23 September 2013 by KrDa (Talk | contribs)

Twin-arginine translocation reporter

This biobrick is a part composed of GFP SF exported to the periplasm by twin-arginine translocation (TAT) signal peptide. In addition this is transcriptionally fused to a RFP with RBS associated.


Usage and Biology

The twin-arginine translocation

The ration of expression between GFP SF and RFP can be tuned by switching out the RBS sequence upstream GFP SF.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1225
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1225
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 149


[edit]
Categories
//cds/membrane/transporter
//classic/reporter/pret
Parameters
signalling_molecule