Help:Glossary

• PoPS (Polymerase Per Second)

Transcription initiation is defined as the number of times that an RNA Polymerase passes from the regulatory region and into the protein coding region of a BioBrick gene. PoPS are measured by introducing a test gene with the same regulatory region but with a CFP protein coding region and measuring the fluorescence.

• Regulatory Region

The canonical model of a BioBrick component asserts that proteins generated as a result of other logical or cellular processes (see Protein Coding Region) as well as other active molecules interact with the computational body of a BioBrick component to produce the output signal. The genetic code of the computational body is represented by a Regulatory Region of DNA.

• Protein Coding Region

A section of DNA that can cause the generation of a specific protein. In BioBricks, this includes the BioBrick prefix and suffix, the Ribosome Binding Site (RBS), the codons for the protein's amino acids, and the stop codon.

• Canonical Model

A simplified model which adequately represents the components and interactions under study. The actual system may or may not be structured like the canonical model.

• BioBrick Component

A portion of a BioBrick device that is available as a BioBrick product. A protein coding region is a BioBrick device if it is available as a BioBrick product and documented in the BioBrick Data Book.

• BioBrick Device

A BioBrick system consisting of one or more BioBrick components that together instantiate a full logical or functional process. Normally, this consists of input protein coding regions that code for the input proteins and a BioBrick computational body that processes the input proteins and generates TIPS at the output sites.