Temporary

Part:BBa_J25500

Designed by: Mackenize Cowell   Group: iGEM06_BU   (2006-07-12)
Revision as of 00:06, 13 July 2006 by Registry (Talk | contribs)

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Light-repressed mCherry (uses photosensor)

The photosensor (M30109) Natalie Kuldell used in her BE.109 course controls expression of mCherry+lva (J06505). This is an intermediate composite part intended to test the viability of our construction of the photosensor. Eventually we would like to control bioluminesence of the Lux system (LuxCDABE) with the photosensor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1702
    Illegal BglII site found at 4190
    Illegal BamHI site found at 65
    Illegal XhoI site found at 2297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1255
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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