Device
Part:BBa_K1210000
Designed by: Zak Stinson Group: iGEM13_Lethbridge (2013-08-02)
LacI-CFP-PK401-YFP
Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Produces a reporter of framshift-induction. If YFP coding region is read in the 0 frame, FRET can be observed. If the YFP coding region is translated in the -1 frame, there will be no FRET.
This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |