Generator
Part:BBa_K1049002:Experience
Designed by: Yohei TAMIAKI Group: iGEM13_KIT-Kyoto (2013-09-19)
Revision as of 02:08, 20 September 2013 by Okaeri007 (Talk | contribs) (→Applications of BBa_K1049002)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1049002
Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis.
User Reviews
UNIQf9b71734bdedb682-partinfo-00000000-QINU UNIQf9b71734bdedb682-partinfo-00000001-QINU