Composite

Part:BBa_K1140006:Design

Designed by: Janssel Reyes del Castillo   Group: iGEM13_UANL_Mty-Mexico   (2013-09-13)
Revision as of 04:07, 14 September 2013 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pTet + 37 oC RNA thermometer + mCherry (LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.


Source

Obtained synthetically. Promoter pTet. Based on a fusion of a RBS and a Riboswitch. RFP with a LVA tag (Further information coming soon).

References