Composite
Part:BBa_J32001:Design
Designed by: Austen Heinz Group: iGEM06_Duke (2006-07-11)
Targeted Localization
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2471
Illegal PstI site found at 2597 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1391
Illegal PstI site found at 2471
Illegal PstI site found at 2597 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1669
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2471
Illegal PstI site found at 2597 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2471
Illegal PstI site found at 2597
Illegal NgoMIV site found at 1079
Illegal NgoMIV site found at 1680
Illegal NgoMIV site found at 2305
Illegal AgeI site found at 2080 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1146
Illegal BsaI.rc site found at 724
Illegal BsaI.rc site found at 1357
Illegal SapI.rc site found at 1561
Design Notes
It is a fusion protein so biobrick prefixes and suffixes have been modified to 10 and 24 bp, removing the bp between the flanking restriction sites and the sequence. Otherwise it would read out of frame with 8bp overlap
Source
The signal sequence PelB and S*Tag sequence came from Novagen. The antibody sequence is for cAb-CEA5 nanobody from Ablynx. The C-terminus of Neisseria gonorrhoeae IgA Protease NCBI accession number AE004969