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Part:BBa_J32006

Designed by: Austen Heinz   Group: iGEM2006_Duke   (2006-07-11)
Revision as of 02:48, 12 July 2006 by Registry (Talk | contribs)

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It is a fusion protein so biobrick prefixes and suffixes have been modified to 10 and 24 bp, removing the bp between the flanking restriction sites and the sequence. Otherwise it would read out of frame with 8bp overlap. Should bind to other cells expressing appropriate antigen

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 364
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 638
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1440
    Illegal PstI site found at 1566
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 649
    Illegal NgoMIV site found at 1274
    Illegal AgeI site found at 1049
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 119
    Illegal BsaI.rc site found at 330
    Illegal SapI.rc site found at 530


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