Regulatory
AraC

Part:BBa_K1088017

Designed by: Patrick Rosendahl Andreassen   Group: iGEM13_SDU-Denmark   (2013-09-16)
Revision as of 13:43, 16 September 2013 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


AraC device (constitutive promoter, RBS and terminator)

Coding region for the LacI protein derived from E. coli. LacI binds to the lac promoter BBa_R0010 and inhibits transcription. IPTG (Isopropyl β-D-1-thiogalactopyranoside) binds to LacI and inhibits its operation, thereby promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None