Plasmid

Part:BBa_K1039016

Designed by: Julia Manalil   Group: iGEM13_Waterloo   (2013-09-16)
Revision as of 21:53, 17 September 2013 by J2manali (Talk | contribs)

HPdO Helper Plasmid

HPdO Helper Plasmid contains the M13 viral genome but is lacking the f1 origin and packaging sequence. It contains the p15A origin of replication and a kanamycin resistance gene (Lin et al., 2011).

References

  1. Lin A, Jimenez J, Derr J, Vera P, Manapat ML, Esvelt KM, Villanueva L, Liu DR, Chen IA: Inhibition of bacterial conjugation by phage M13 and its Protein g3p: quantitative analysis and model. PLoS One 2011, 6: e19991.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 8168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5916
    Illegal PstI site found at 8168
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2622
    Illegal XhoI site found at 8010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 8168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 8168
    Illegal AgeI site found at 5956
    Illegal AgeI site found at 6280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8173
    Illegal BsaI.rc site found at 6985


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Categories
Parameters
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