Coding
EbfS Aa

Part:BBa_K1060002:Design

Designed by: Ingmar Claes, Lukas Marcelis, Su Wang   Group: iGEM13_KU_Leuven   (2013-09-17)
Revision as of 11:00, 17 September 2013 by Registry (Talk | contribs)

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EBF synthase from Artemisia annua


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1306
    Illegal BamHI site found at 615
    Illegal XhoI site found at 577
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 187


Design Notes

An EcoRI site (513A>C)was removed by site directed mutagenesis


Source

Artemisia annua genomic DNA (Picaud et al. 2005)

References