Composite

Part:BBa_K1149004

Designed by: Margarita Kopniczky   Group: iGEM13_Imperial_College   (2013-09-13)
Revision as of 19:14, 15 September 2013 by Margarita K (Talk | contribs)

pelB-pueB expression

PUR degradation enzyme from

PUR_esterase_theoretical_pathway_2.jpg

references:

[http://www.sciencedirect.com/science/article/pii/S0964830501000427 Cloning, nucleotide sequencing and characterization of a polyurethanase gene (pueB) from Pseudomonas chlororaphis (2001)] <p/> <p> [http://www.sciencedirect.com/science/article/pii/S0964830502000690 secretion and expression in E.coli]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BglII site found at 1213
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 407
    Illegal AgeI site found at 752
    Illegal AgeI site found at 1241
    Illegal AgeI site found at 1517
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None