Reporter

Part:BBa_K1114505

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-12)
Revision as of 00:50, 13 September 2013 by Registry (Talk | contribs)

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MoClo Level 1 RFP reporter with AE fusion sites

pJ04B16Rm_AE This is a MoClo Level 1 reporting device containing the Level 0 parts J23104_AB, BCD16_BC, E1010m_CD, and B0015_DE within the DVL1_AE backbone. The flanking fusion sites are A on the 5' side and E on the 3' side of the insert. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1238
    Illegal XbaI site found at 1265
    Illegal SpeI site found at 994
    Illegal PstI site found at 1277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1238
    Illegal NheI site found at 11
    Illegal NheI site found at 34
    Illegal SpeI site found at 994
    Illegal PstI site found at 1277
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1238
    Illegal BamHI site found at 1259
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1238
    Illegal XbaI site found at 1265
    Illegal SpeI site found at 994
    Illegal PstI site found at 1277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1238
    Illegal XbaI site found at 1265
    Illegal SpeI site found at 994
    Illegal PstI site found at 1277
    Illegal AgeI site found at 710
    Illegal AgeI site found at 822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1502
    Illegal BsaI.rc site found at 1014


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