RBS

Part:BBa_K1114107:Design

Designed by: Traci Haddock   Group: iGEM13_BostonU   (2013-09-07)
Revision as of 19:59, 7 September 2013 by THaddock (Talk | contribs) (→‎Source)

Bicistronic Design RBS 2, MoClo Format with BC fusion sites



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

This is the MoClo formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See BBa_K783055.

Source

This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= "http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html">Mutalik, et al.</a>

References

<a href= “http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html”?>Mutalik, et al.</a>