Regulatory

Part:BBa_K1114011:Design

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-07)
Revision as of 17:57, 7 September 2013 by Jawtry (Talk | contribs) (Created page with "<partinfo> BBa_K1114011 </partinfo> <partinfo> BBa_K1114011 SequenceAndFeatures </partinfo> <html> This is the <a href=”http://2013.igem.org/Team:BostonU/MoCloChara”>MoClo...")

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This is the MoClo formatted part of BBa_J23108 with AB fusion sites. See the BBa_J23108 page for full information on the part.

This is a MoClo level 0 promoter with a flanking site A on the 5' side and site B on the 3' side of the part.

The fusion site letters refer to 4bp fusion sites: A=GGAG; B=TACT; C=AATG; D=AGGT; E=GCTT; F = CGCT; G=TGCC; H=ACTA.

Summary of modifications from original part: Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.