Composite

Part:BBa_K1036003

Designed by: Xin Guo, Xi Xi   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 09:05, 3 September 2013 by Cproud (Talk | contribs) (→‎Construction)

lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP(lva tag).

Usage and Biology

Protocol

Construction

This part is constructed with quorum sensing promoter luxR, GFP with LVA tag, and LuxI. Via digestion and ligation, these parts are ligated as a composite.

Microfluid

SDS page

Experimental Data

SDS page, microfluid, and gel image of construction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3909
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828
    Illegal BsaI.rc site found at 2086
    Illegal BsaI.rc site found at 3189


[edit]
Categories
Parameters
None