Composite

Part:BBa_K1036003

Designed by: Xin Guo, Xi Xi   Group: iGEM13_XMU-China   (2013-08-27)
Revision as of 08:53, 3 September 2013 by Cproud (Talk | contribs) (→‎Protocol)

lux pL controlled luxR with lux pR controlled gfp (LVA-tag)

Lux pL controlled LuxR + lux pR autoinducing LuxI (lva tag) + lux pR controlled GFP(lva tag).

Usage and Biology

Protocol

Construction

Microfluid

SDS page

Experimental Data

SDS page, microfluid, and gel image of construction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3909
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1828
    Illegal BsaI.rc site found at 2086
    Illegal BsaI.rc site found at 3189


[edit]
Categories
Parameters
None