Coding

Part:BBa_K1218011:Design

Designed by: Sophia Liang   Group: iGEM13_Stanford-Brown   (2013-08-29)
Revision as of 20:00, 29 August 2013 by Sophia liang (Talk | contribs) (→‎Source)


Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1642
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3921
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4863
    Illegal BsaI.rc site found at 4840


Design Notes

Mutagenesis to remove internal EcoRI site


Source

CRISPR-Cas system from streptococcus pyogenes; cloned from plasmids obtained from Bikard et al.

References

http://nar.oxfordjournals.org/content/41/15/7429