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Part:BBa_K1185001:Design

Designed by: Vincent Leonardo   Group: iGEM13_Newcastle   (2013-08-29)
Revision as of 11:44, 29 August 2013 by VLeonardo (Talk | contribs) (→‎Design Notes)


HBsu-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 339


Design Notes

There are no illegal restriction sites in this BioBrick. As the hbs gene is found naturally in B. subtilis there is no need for codon optimization. We chose the linker sequence as it is flexible and so does not interfere with the function of the fusion protein.

Source

The source of the HBsu coding sequence was the B. subtilis strain 168 genome, SwissProtTM accession number: P08821. The sfGFP sequence was sourced from BBa_E0040 and the linker from: BBa_K105012.

References