Reporter

Part:BBa_K1088002

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-07-09)
Revision as of 16:02, 9 July 2013 by Registry (Talk | contribs)

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Reporter system to quantify protein expression of B. sub 1-deoxyxylulose-5-phosphate synthase (Dxs)

We constructed a system to quantify the protein expression of our desired gene, Dxs, by using the amilCP reporter. The system is under transcriptional control by a sigma 70 dependent promoter and we have used the terminator present in the pSB1C3 plasmid. The Dxs gene is translationally fused to the amilCP gene and therefore the stopcodon of dxs have been removed. The Dxs gene was amplified with primers that carried both RBS and the promoter in its overhang. Also a glycine linker was added between dxs and amilCP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1019
    Illegal AgeI site found at 912
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 861
    Illegal SapI.rc site found at 1560


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