Plasmid_Backbone

Part:BBa_M36093:Design

Designed by: Joseph Shih   Group: Stanford BIOE44 - S11   (2013-05-08)
Revision as of 17:43, 18 June 2013 by Jdshih (Talk | contribs) (→‎Source)


S. cerevisiae sensor test rig


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4296
    Illegal XbaI site found at 5221
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4296
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4296
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4296
    Illegal XbaI site found at 5221
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4296
    Illegal XbaI site found at 5221
    Illegal AgeI site found at 1827
    Illegal AgeI site found at 3909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Has S. cerevisiae specific LEU selection and 2 um origin of replication allowing for 50-100 copies of the plasmid in yeast cells.

Source

Vector is based off of pJ1201-03c plasmid developed by DNA2.0.

Note: original pJ1201-03c plasmid does not have a yeast Kozak sequence. It has been added to this vector; student designed yeast sensors being synthesized by DNA2.0 will have the yeast Kozak automatically added to the 3' end of the design.

References