Generator

Part:BBa_K511805

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)
Revision as of 04:19, 29 September 2011 by Chsuqed (Talk | contribs)

Repressible Yellow Fluorescence Generator (Hef1a-LacO-EYFP-4xFF4) MammoBlock Device

This MammoBlock composite device produces the yellow fluorescent protein EYFP-FF4x4 at a low, constitutive level; in addition, this device can be inhibited by LacI variants.



(1A) This scatter plot shows the distribution of the Hek293 population after it was transfected with the following DNA parts: Hef1a-LacO:eYFP and Hef1a:mKate, both of which were constitutively active, expressing yellow and red fluorescent proteins respectively. We observe a distinct shift of approximately 83% of the population in their eYFP fluorescence (FITC channel), while we observe a 69% shift in mKate fluorescence (PE-TexasRed channel). (1C) The negative control sample was transfected with plasmids containing no functional promotor-gene pairs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1502
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1502
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BamHI site found at 255
    Illegal XhoI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1502
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal PstI site found at 1502
    Illegal NgoMIV site found at 804
    Illegal AgeI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]


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