Reporter

Part:BBa_K511101

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)
Revision as of 03:55, 29 September 2011 by Grantrob (Talk | contribs)

EYFP-FF4x4 MammoBlock

This part is an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow. Additionally, this protein has four binding sites for the FF4 micro RNA (miRNA). In the presence of FF4 miRNA, translation of this protein is markedly attenuated.

Figures 1 and 2 show typical levels of EYFP-FF4x4-mediated fluorescence following transfection of HEK-293 cells with EYFP-FF4x4 driven by the Hef1a-LacOid low-level constitutive promoter.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aEYFP-FF4x4 MammoBlock