Part:BBa_M36071
K12 e.coli native Manganese Superoxide Dismutase
Coding sequence for the enzyme Mn-SOD or SoDA. This protein catalyzes the dismutation of superoxide, a free radical. Sequence found by converting the amino acid sequence of Mn-SOD protein native to Escherichia coli, the organism for which it is also optimized for.
Usage and Biology
Superoxide dismutase cataylyzes the dismutation of superoxide. Superoxide is produced naturally in the respiration process. It causes damage to the cell by altering lipid structure and breaking down proteins among other negative effects. This gene provides the protein to transform two superoxide molecules into hydrogen peroxide and oxygen.
Design Notes
This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico. We optimized our sequence in DNA 2.0's Gene Designer by increasing GC content and minimizing repeats. We accomplished this by altering basepairs without altering amino acids.
Source
This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 346
Illegal XhoI site found at 43
Illegal XhoI site found at 127 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 346
Illegal PstI site found at 283
Illegal PstI site found at 593
Illegal NgoMIV site found at 542 - 1000COMPATIBLE WITH RFC[1000]
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