Coding
SoDA

Part:BBa_M36071

Designed by: Alex Rosay, Justin Diep, Ben Blankenmeister   Group: Stanford BIOE44 - S11   (2012-12-07)
Revision as of 10:59, 7 December 2012 by Arosay (Talk | contribs)

K12 e.coli native Manganese Superoxide Dismutase

Coding sequence for the enzyme Mn-SOD. This protein catalyzes the dismutation of superoxide, a free radical. Sequence found by converting the amino acid sequence of Mn-SOD protein native to Escherichia coli, the organism for which it is also optimized for.

Usage and Biology

Superoxide dismutase cataylyzes the dismutation of superoxide. Superoxide is produced naturally in the respiration process. It causes damage to the cell by altering lipid structure and breaking down proteins among other negative effects.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 346
    Illegal XhoI site found at 43
    Illegal XhoI site found at 127
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 346
    Illegal PstI site found at 283
    Illegal PstI site found at 593
    Illegal NgoMIV site found at 542
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None