Coding
RhaR
Part:BBa_K902069:Design
Designed by: Chris Wintersinger Group: iGEM12_Calgary (2012-10-02)
RhaR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 559
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
RhaR is present at basal levels in cells. It binds with the inducer rhamnose to upregulate the rhaSR operon off the pRha rhamnose promoter. In turn, more RhaR and RhaS is produced, where RhaS can then activate the rhaBAD operon on the opposite side of the rhamnose promoter (Egan & Schleif, 1993).
Source
PCR amplified from Top Ten E. coli using Kapa Hi-Fi polymerase.
References
Egan, S. M., Schleif, R. F., & others. (1993). A regulatory cascade in the induction of rhaBAD. Journal of Molecular Biology, 234(1), 87-98.