Translational_Unit
Part:BBa_K914018
Designed by: Jean Cury Group: iGEM12_Paris_Bettencourt (2012-10-25)
P1003** Kan resistant gene with 2 Amber Codon
The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//biosafety/semantic_containment
//cds/selectionmarker/antibioticresistance
//cds/selectionmarker/antibioticresistance
None |