Translational_Unit

Part:BBa_K914018

Designed by: Jean Cury   Group: iGEM12_Paris_Bettencourt   (2012-10-25)
Revision as of 13:32, 25 October 2012 by Registry (Talk | contribs)

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P1003** Kan resistant gene with 2 Amber Codon

The amber mutations avoid the expression of the P1003 gene even at usual concentration that prevent the expression of kanamycin resistance, although one mutation (BBa_K914009) permits some leakage in translation for this gene. This mutation is rescued with the tRNA amber suppressor supD (BBa_K914000). The idea here is to prevent the kanamycin gene resistance to be expressed in case horizontal gene transfer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//biosafety/semantic_containment
//cds/selectionmarker/antibioticresistance
Parameters
None