Part:BBa_K792010

Yeast exportable Trp-rich peptide w/enhanced import (1)

This composite device produces and exports a Tryptophan rich peptide, that is import enhanced (thanks to a trojan peptide). This device is intended to be used with Yeast chassis, so it is composed by subparts designed also to be use in yeast.

The high level structure of the device is:

• Kozak consensus sequence for initiation of translation (from yeast α-factor mating pheromone (MFα1))
• Signal peptide that targets the product of the gene for secretion (also from yeast α-factor mating pheromone (MFα1))
• Trojan peptide, to increase internalization in target cell (from HIV TAT penetratin)
• Payload: this is the exported Tryptophan rich domain of the protein (a TrpZipper)

The device's sequence has inherited some useful restriction sites (BamHI & HindIII) from the parts it's made of.

Important note:

this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from BB assembly standard.

See also:	Another Trp-rich peptide export and enhanced import composite device Part:BBa_K792012.
	Similar design His-rich export and enhanced import composite device Part:BBa_K792009.

The device was originally designed and built to be used in [http://2012.igem.org/Team:Buenos_Aires iGEM BsAs 2012 project] to implement a cross-feeding regulatory system between two different yeast strains with specific auxotrophy. [http://2012.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details. Also feel free to contact us for any question regarding this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]