Generator
pRha-B0034

Part:BBa_K902067:Design

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-10-02)
Revision as of 03:27, 27 October 2012 by Stephanie0101 (Talk | contribs) (→‎Source)

Rhamnose promoter with S7 micrococal nuclease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed as part of a kill switch for 2012 iGEM Calgary project. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for more details about our kill system. This circuit needs to be grown up on special LB plates containing >0.2% glucose to repress this system successfully.

Source

The pRha promoter and S7 nuclease were commercially synthesized. The RBS was taken from the Parts Registry. This part was constructed using the basic biobrick assembly method.

References

Dingwall C, Lomonossoff GP, Laskey RA. High sequence specificity of micrococcal nuclease. Nucleic Acids Res 1981 Jun 25;9(12):2659-2673.