Coding
BAMT

Part:BBa_J45002:Design

Designed by: André Green II   Group: iGEM06_MIT   (2006-06-07)
Revision as of 23:35, 13 June 2006 by Drephd (Talk | contribs) (→‎Design Notes)


SAM:benzoic acid carboxyl methyltransferase; converts benzoic acid to methyl benzoate (floral odor)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • There are two Met codons at the N-terminus of the protein (putative codons 1 and 4). It is believed that the first Met codon is the actual start of the protein. Reference:

<biblio>

  1. Dudareva pmid=11051108

</biblio>

  • Bases 1-18 of the original A. majus sequence (gccggacgccaaagaaaa) were deleted. Thus, this sequence begins with the first start codon.
  • A silent mutation (A -> G) was made at base 702 to eliminate an SpeI cut site.
  • The original stope (TGA at bp ___) was deleted and a double TAA stop was inserted

Source

Genbank accession number(s): AF198492

We got this cDNA from N Dudareva at Purdue.

References