Generator

Part:BBa_K814000

Designed by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn   Group: iGEM12_Minnesota   (2012-10-03)
Revision as of 18:50, 3 October 2012 by Registry (Talk | contribs)

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dehydroquinate synthase (DHQS) generator

Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate.

This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 250
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 250
    Illegal NotI site found at 1373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 250
    Illegal BglII site found at 134
    Illegal XhoI site found at 1381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 250
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 250
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None