Generator
Part:BBa_K814000
Designed by: Nolan Kennedy, Michelle Goettge, Grayson Wawrzyn Group: iGEM12_Minnesota (2012-10-03)
dehydroquinate synthase (DHQS) generator
Our research has focused on two novel biosynthetic pathways found in two distinct algal species. A pathway ending in the production of two UV-protective compounds, shinorine and mycosporine-glycine, was cloned from Anabaena varibalis. DHQS catalyzes the first step in the pathway, converting sedoheptulose-7-phosphate into dehydroquinate.
This part includes a modified constitutive lac promoter (lacP'), and RBS and the open reading frame of DHQS. This part can be used to express DHQS in E. coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 250
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 250
Illegal NotI site found at 1373 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 250
Illegal BglII site found at 134
Illegal XhoI site found at 1381 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 250
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 250
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |