Composite

Part:BBa_K732002

Designed by: Joshua Fass   Group: iGEM12_Virginia   (2012-10-01)
Revision as of 21:00, 1 October 2012 by Registry (Talk | contribs)

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synCG-β + Promoter + RBS

This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-β (K732001), and should strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 224
    Illegal NgoMIV site found at 268
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prc
//function/reporter
Parameters
None