Composite
Part:BBa_K732002
Designed by: Joshua Fass Group: iGEM12_Virginia (2012-10-01)
synCG-β + Promoter + RBS
This construct contains a strong constitutive promoter (J23119) as well as an RBS (B0034) upstream of synCG-β (K732001), and should strongly express a synthetic version of the beta subunit of human chorionic gonadotropin.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 224
Illegal NgoMIV site found at 268 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/generator/prc
//function/reporter
//function/reporter
None |