Coding
synCG

Part:BBa_K732001:Experience

Designed by: Joshua Fass   Group: iGEM12_Virginia   (2012-10-01)
Revision as of 04:01, 10 October 2012 by J.fass (Talk | contribs) (→‎Applications of BBa_K732001)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K732001

One application of this component is detection by pregnancy test.

When heterogeneous mixture of E. coli expressing BBa_K732001 and BBa_K732002 was lysed by either sonication or T7 infection, re-folded using a literature-based protocol, and applied to NewChoice pregnancy tests (purchased from Dollar Tree), positive results were visible.

Note that (a) there was some coloration around the positive band, and (b) the pregnancy tests were left overnight for bands to become visible. We hypothesize that the delayed read-time is necessary because the folding solution interferes with band visibility.

Virginia 2012 Oct 2 Preg Test Results.jpg

User Reviews

UNIQ3812de19085a2a7a-partinfo-00000000-QINU UNIQ3812de19085a2a7a-partinfo-00000001-QINU