Coding
amilCP

Part:BBa_K592009:Experience

Designed by: Lei Sun   Group: iGEM11_Uppsala-Sweden   (2011-09-18)
Revision as of 03:48, 27 September 2012 by RenskevR (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K592009

User Reviews

UNIQ4d316acb7d55472d-partinfo-00000000-QINU UNIQ4d316acb7d55472d-partinfo-00000001-QINU

BBa_K592009 iGEM Groningen 2012

Our team has managed to couple this biobrick part with our promoters: alsT, fnr, and sboA. The cloning was done in BBa_K818000 (plasmid backbone for B. subtilis, engineered by team Groningen 2012), to allow color expression in B. subtilis. We utilized a strong RBS BBa_B0034 for pigment expression in E. coli and B. subtilis.

The purple/blue colour was strongly visible in E.coli without any induction, while the expression in B. subtilis was more subtle (B. subtilis colony looks slightly blue on the plate agar).

After induction of the promoter before AmilCP, B. subtilis also turned clearly purple/blue. Please have a look at the page of BBa_K818400 for more information.

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