Composite

Part:BBa_K887002:Experience

Designed by: Chung, Chia-Jung   Group: iGEM12_NCTU_Formosa   (2012-09-21)
Revision as of 01:06, 6 October 2012 by Zcc (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K887002

1.Isobutanol Tolerance

We did an experiment to prove the isobutanol is truly toxic to the E.coli. The data shows that the higher concentration of the isobutanol was in the medium, the lower OD600 value could be obtained.

Isobutanol tolerance.jpg
Figure 1.Activate our E.coli overnight. Then, transfer it into the new medium with the microaerobic environment until OD600 reached 0.2. After that, measure the OD600 every 4 hours.
When the temperature reaches 37.jpg
Figure 2. When the temperature reaches 37℃, tetR will be expressed and TetR will inhibit ptet.KivD can't be translated.

2.Our system Do Work
MEFL.jpg
Figure 3.Mark the second circuit with the fluorescent protein to test the expression of kivD enzyme.

According to Figure 3, our low temperature release system do truly work !We used the fluorescent protein to mark the second circuit of our biobrick. The data tells us that kivD enzyme under 37℃ environment had the lower expression than under 30℃ and the 25℃ environment.

3.27°C culture condition
Transfer to 27.jpg
Figure 4.Transfer to different temperature at different timing. We inoculated our E.coli in the 37℃ environment until OD600 up to 0.2 , which we set as 0 hour. Then, E.coli was transferred into 27℃ after being 0 hour, 12 hours , 16 hours, 20 hours and 24 hours.

4.32°C culture condition
Transfer to 32.jpg
Figure 5.Transfer to different temperature at different timing. We inoculated our E.coli in the 37℃ environment until OD600 up to 0.2 , which we set as 0 hour. Then, E.coli was transferred into 32℃ after being 0 hour, 12 hours , 16 hours, 20 hours and 24 hours.

5.42°C culture condition
Transfer to 42.jpg
Figure 6.Transfer to different temperature at different timing. We inoculated our E.coli in the 37℃ environment until OD600 up to 0.2 , which we set as 0 hour. Then, E.coli was transferred into 42℃ after being 0 hour, 12 hours , 16 hours, 20 hours and 24 hours.

User Reviews

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