Plasmid_Backbone

Part:BBa_K914012

Designed by: Jean Cury   Group: iGEM12_Paris_Bettencourt   (2012-10-05)
Revision as of 22:50, 5 October 2012 by Registry (Talk | contribs)

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pSB1A2 with 1 amber mutation

pSB1A2 have a double mutation in the 80th serine (TCC->TAG) of the APr gene. This plasmid need a tRNA amber suppressor to express this gene. So in case of horizontal gene transfer this gene won't be expressed and then the plasmid will be lost more easily.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2064
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2058
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2058
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2058
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2073
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1097


[edit]
Categories
//biosafety/semantic_containment
Parameters
None