Reporter
RO

Part:BBa_K838001:Design

Designed by: Nicolas Gobet   Group: iGEM12_EPF-Lausanne   (2012-09-22)
Revision as of 18:01, 4 October 2012 by Mcheraka (Talk | contribs) (→‎Design Notes)

LovTAP readout


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 424
    Illegal BglII site found at 381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 424
    Illegal SpeI site found at 295
    Illegal SpeI site found at 303
    Illegal NgoMIV site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part contains two illegal SpeI sites and one illegal EcoRI site. We planned on performing site directed mutagenesis but unfortunately the kit we needed didn't arrive on time due to issues and the attempts we had weren't successful yet.

The part can still be cut out with XbaI and PstI.

Source

Nicolas Gobet

References