Coding

Part:BBa_K898890:Design

Designed by: Hsiao-Han Chen. Anthony Chau. Uros Kuzmanovic. Zefeng Wang.   Group: iGEM12_UIUC_Illinois   (2012-09-30)
Revision as of 03:35, 4 October 2012 by Angelalovestheworld (Talk | contribs) (→‎References)

wtPUF+PIN


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1297
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization Method

PUFPINreporter.png


This figure includes the resulting PUF-PIN proteins interacting with the binding sites. The wild type binding site placed in between RBS and YFP, is represented by the orange oval. The yellow rounded rectangle represents the YFP reporter gene.

Source

The PUF domain from human Pum1 (residue 828-1176) and the PIN domain from human Smg6 (residue 1238-1421)

References

Professor Zefeng Wang, University of North Carolina- Chapel Hill

http://www.med.unc.edu/pharm/people/primaryfaculty/zefeng-wang/wang