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Composite

Part:BBa_K781006

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-03)
Revision as of 06:10, 3 October 2012 by Registry (Talk | contribs)

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[R0010][B0034] - RFP-FliC Deletion Chimera

This is the modified RFP protein obtained from J04450, that has been incorporated into the E. coli flagellin as a deletion.

This part can be used as a template for making other PCR overlap insertions. After transforming the product, and digesting the template plasmid with Dpn1, any template that has not been successfully digested will form red colonies.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 529
    Illegal AgeI site found at 1362
    Illegal AgeI site found at 1474
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/a[R0010][B0034] - RFP-FliC Deletion Chimera