Composite

Part:BBa_K902007:Design

Designed by: Emily Hicks   Group: iGEM12_Calgary   (2012-09-06)
Revision as of 03:49, 7 September 2012 by Registry (Talk | contribs)

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Beta-D-glucoside glucohydrolase (bglX) with strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1639
    Illegal AgeI site found at 1861
    Illegal AgeI site found at 2050
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An illegal pstI site was removed from the 15th and 16th amino acid positions via site directed mutagenesis.


Source

The beta-D-glucoside glucohydrolase (bglX) gene was amplified from TOP10 E. coli via polymerase chain reaction.

References