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Part:BBa_K844012:Design

Designed by: Andrea Halling   Group: iGEM12_Utah_State   (2012-10-03)
Revision as of 02:21, 4 October 2012 by Ryanputman (Talk | contribs) (→‎Design Notes)

tRNA expression cassette for spider silk "F" proteins


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When working with tRNA expression systems, you cannot have scar sites between the promoter and the gene, or between the gene and the terminator. As such, the entire sequence was synthesized without scars. At minimum, no scars can be internal to an individual tRNA expression unit, but can exist between different complete expression units. Note: the Arg(TCT) tRNA is from Escherichia_coli_O157H7 since the K12 ARG tRNA had an internal PstI site.

Source

This part was chemically synthesized but based upon E. coli genomic DNA sequences, and BioBrick parts BBa_K844010 and BBa_K844011.

References