Coding
Part:BBa_K902004:Design
Designed by: Emily Hicks Group: iGEM12_Calgary (2012-08-29)
Periplasmic beta-D-glucoside glucohydrolase (bglX)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1621
Illegal AgeI site found at 1843
Illegal AgeI site found at 2032 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
An illegal pstI site at the 15th and 16th amino acid position was mutated out via site-directed mutagenesis.
Source
This gene was amplified from the genome of E. coli via polymerase chain reaction.