Coding

Part:BBa_K936030

Designed by: Sergio Castro Waqued   Group: iGEM12_UC_Davis   (2012-10-03)
Revision as of 08:24, 3 October 2012 by Registry (Talk | contribs)

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LC Cutinase mutant T96A

Mutant of the Polyetheylene Terephtalate degrading protein LC Cutinase (Bba_K936000). The threonine at the 96th amino acid position is changed to an alanine. This part was created using site directed mutagenesis to change an "ACG" codon to a "GCA" codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 600
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None