Coding

Part:BBa_K936014

Designed by: Nicholas Csicsery   Group: iGEM12_UC_Davis   (2012-08-09)
Revision as of 16:50, 9 August 2012 by Registry (Talk | contribs)

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pelB-LC-Cutinase with polyhistidine-tag

pelB leader sequence attached to enzyme LC-Cutinase protein coding sequence in order to allow the cell to bring the enzyme to the periplasm for easier secretion. The polyhistidine-tag allows for us to purify the produced enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 666
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None