Coding

Part:BBa_K842011

Designed by: Sean P. Curran, Percy Genyk, Ellen Park, Rebecca, Gao, Stephan Genyk, Megan Bernstein, Rachel Kohan, Eric Siryj, Luke Quinto   Group: iGEM12_USC   (2012-10-02)
Revision as of 01:20, 4 October 2012 by Rkohan (Talk | contribs)

USC mota.gif

motA is a structural protein for the stationary element of the flagella motor. The protein works in conjunction with motB to form this structure, which then in turn controls the flagella’s rotation. Used for reconstitution of flagella apparatus in E. coli B strains.

Cloning and Uses This part was generated via PCR from the E. coli strain DH5α and cloned into the vector pSB1C3. The protein works in conjunction with motB to form the stationary base of the flagella motor, which then in turn controls the flagella’s rotation. This part can be used in the complete recreation of an E. coli’s genetic flageller pathway.

















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 323
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 829


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Categories
Parameters
None